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KMID : 0376419960200020177
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Chonbuk University Medical Journal
1996 Volume.20 No. 2 p.177 ~ p.184
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Study on In Vitro Muaturation anf Role of Cumulus Cells on the Ferilization of Mouse Oocytes
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이희섭
류철희/이기술/오병찬/조성남/진소자/두재균/김관식/김동덕
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Abstract
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The time course and conditions necessary for oocyte maturation and the role fo cumulus cells on fertilization in vitro were studied in the mouse. Nuclear maturation of cultured oocytes was evaluated by analyzing chromosomal configurations.
Isolated
oocytes from cumulus-oocyte complex were matured spontaneously in vitro, and 81% of oocytes developed to the metaphase II after 24 hours incubation. Cumulus-oocyte complex matured in vitro, and 86% of those developed to the metaphase II after 24
hours.
The fertilization frequency of isolated oocytes in hyaluronidase was similar in both +HCG and -HCG culture medium (29.2% and 21.7%, respectively). However the fertilization frequency of cumulus-oocyte complex was greater than that of isolated
oocytes,
and the fertilization frequency of cumulus-oocyte complex cultured in +HCG culture medium was lower than that cultured in -HCG culture medium (36.4% and 58%, respectively).
It is concluded that frequency of spontaneous maturation of isolated oocyte was similar to that of cumulus-oocyte complex, and that the presence of cumulus cells during fertilization may affect the oocyte's fertilization.
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KEYWORD
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